HEMOPHILIA GENNK CYP3A5 HLA-B27 Thalassemia NK-CELL ACTIVITY TEST

CYP3A5

OVERVIEW CYP3A5 TEST

Tacrolimus dosing aid gene test is a CYP3A5 genotyping test that helps determine the appropriate dose, individualized in treatment, helps to quickly achieve the target C0 concentration immediately after transplantation, limiting maximize the rate of acute rejection, improve the effectiveness and safety of treatment.
There are currently many anti-immunosuppressive drugs used after transplantation but Tacrolimus is the drug of first choice according to the guidelines of the Vietnam Organ Transplantation Association (VSOT) 2017.

Tacrilimus common name FK506 has

- The therapeutic range is narrow, so Ctrough concentrations (C0, Ctrough) are recommended to monitor concentrations and adjust daily dose;

- CYP3A5 genotype affects the rate of metabolism of Tacrolimus;

- Reaching the Ctrough target early reduces the risk of acute rejection for patients in the first 3 months: 8-12 ng/ml or 5-10 ng/ml depending on the transplant center.

WHY SHOULD THIS TEST WORK?

In the world, there are many studies demonstrating that CYP3A5 genotyping in order to determine the dose of Tacrolimus after transplantation without taking time to detect the dose early to achieve C0 concentration and to limit the risk of acute rejection in the first 15 days, increase the success of the post-transplant, reduce the side effects of the drug.

+ The international guideline of the CPIC association regulates the metabolic rate of the CYP3A5 genotype and the direction of dose adjustment of TAC for CYP3A5 expressor, CYP3A5 non-expressor.

CYP3A5 GENE TEST PROCESS IN GENTIS

DNA extraction.
Amplify the gene region containing variants on the CYP3A5 gene by PCR.
Sequencing genes on the next generation gene sequencing system NGS (Illumina, USA).

CYP3A5 GENE TESTING AT GENTIS

Purpose

Identification of CYP3A5*1 (wild type), CYP3A5*3 (6986 T>C), CYP3A5*6 (14690 C>T) and CYP3A5*7 (27131_27132 insA) variants on the CYP3A5 gene.
The results of this gene polymorphism will be used to determine the dose of tacrolimus.

Sample used: The sample used for the test is 1 - 3 ml of whole blood contained in an EDTA anticoagulation tube

Technology used: Sequencing region carrying variant on CYP3A5 gene by PCR technique on automatic gene sequencing system ABI3130xl (USA)

Time to return results: from 3 days